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The publisher’s final edited version of this article is available at Chem Soc Rev See other articles in PMC that cite the published article. Abstract Fluorescence provides a mechanism for achieving contrast in biological imaging that enables investigations of molecular structure, dynamics, and function at high spatial and temporal resolution. Small-molecule organic fluorophores have proven essential for such efforts and are widely used in advanced applications such as single-molecule and super-resolution microscopy. Yet, organic fluorophores, like all fluorescent species, exhibit instabilities in their emission characteristics, including blinking and photobleaching that limit their utility and performance. Here, we review the photophysics and photochemistry of organic fluorophores as they pertain to mitigating such instabilities, with a specific focus on the development of stabilized fluorophores through derivatization. Self-healing organic fluorophores, wherein the triplet state is intramolecularly quenched by a covalently attached protective agent, exhibit markedly improved photostabilities. Introduction Fluorescence imaging, which affords high specificity and imaging contrast, 1 has proven to be an indispensable tool for advancing our understanding of biological systems. Extrinsic fluorophores have therefore become essential imaging tools. Extrinsic fluorophores spanning the visible spectrum are now available that can be specifically attached to almost any biomolecule of interest. These probes can thus serve as versatile messengers of dynamic and functional information in a diverse array of systems that would otherwise be hidden.

The Parrot and Blue Lake Labels

After all, what wasn’t insane about Death popping up every few minutes, muttering nonsense about “his master,” or getting sucked into a lamp, and bound to a year-old Ninja Wannabe who thought she was a magical Genie? It was strange when Naruto really thought about it. For three years, she missed her home but the moment she came to accept her fate, was the moment that her past decided to come back and haunt her.

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At the special meeting, shareholders will be asked to take the following actions: EMC will transact no other business at the special meeting except such business as may properly be brought before the special meeting or any adjournment or postponement thereof. Only holders of record of EMC common stock as of the record date are entitled to notice of, and to vote at, the special meeting and any adjournment or postponement thereof.

You can obtain this evidence from your broker, bank, trust company or other nominee or intermediary, referred to as a nominee or intermediary, typically in the form of your most recent monthly statement. Approval of the merger agreement requires the affirmative vote, in person or by proxy, of holders of a majority of the outstanding shares of EMC common stock entitled to vote as of the record date for the special meeting.

The approval, on a non-binding, advisory basis, of the compensation payments that will or may be paid Table of Contents by EMC to its named executive officers in connection with the merger requires the affirmative vote, in person or by proxy, of holders of a majority of the shares of EMC common stock represented at the special meeting and entitled to vote thereon. Under the Massachusetts Business Corporation Act, referred to as the MBCA, EMC is required to state whether it has concluded that EMC shareholders are, are not or may be entitled to assert appraisal rights, which are generally available to shareholders of a merging Massachusetts corporation under Section The relevant provisions of the MBCA have not been the subject of judicial interpretation and EMC and Denali reserve the right to contest the validity and availability of any purported demand for appraisal rights in connection with the merger.

In this regard, Denali has indicated that in any appraisal proceeding it will assert, and will cause EMC as its wholly owned subsidiary following completion of the merger to assert, that an exception to appraisal rights is applicable to the merger. Your vote is very important. If your shares are held in the name of a nominee or intermediary, please follow the instructions on the voting instruction card furnished by the record holder. In addition, it constitutes a notice of meeting with respect to the special meeting of EMC shareholders.

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Early life[ edit ] Aikman was born on November 21, He grew up in Cerritos, California. At the age of 12, Aikman’s family moved to Henryetta, Oklahoma , where he played football at Henryetta High School, where he would earn All-State honors. In , his first full season as a collegiate starter, [7] Aikman led the Sooners to victories over Minnesota , Kansas State , and 17 Texas in the Red River Shootout before losing to the Miami Hurricanes as he left the game with a broken leg.

He also lost to his future teammate, Michael Irvin and future head coach, Jimmy Johnson , who also scouted him when he was the head coach of Oklahoma State. Switzer and offensive coordinator Jim Donnan were forced to switch back to the wishbone offense under freshman quarterback Jamelle Holieway. The team went on to win the National Championship.

He had to sit out one year due to college transfer rules but went on to lead the Bruins to a record over two seasons. Aikman led the Bruins to a 10—2 record and the Aloha Bowl, where they beat the Florida Gators

The Code of Trust

This increase was primarily associated with continued growth in the Regulated Businesses driven mainly by infrastructure investment, acquisitions and organic growth, combined with growth in the Market-based Businesses mainly from the Homeowner Services Group. These increases were partially offset by warmer weather in and two discrete tax adjustments recorded at the parent company associated with legislative changes in New York and Illinois impacting state tax apportionment.

The extent to which requested rate increases will be granted by the applicable regulatory agencies will vary. The increase was primarily driven by growth in the Homeowner Services Group through customer growth and price increases for certain customers. The increase was driven by growth in the Homeowner Services Group through customer growth and price increases for certain customers partially offset by lower capital projects in the Military Services Group from lower military budgets and completion of a project at Fort Polk mid-year Set forth in this release is a table that reconciles adjusted net income, as well as Adjusted EPS, both for historical and guidance purposes, to the most directly comparable GAAP financial measure.

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On the eve of the Civil War, Paul Cameron and his siblings owned over one thousand slaves and nearly thirty thousand acres of plantation land in Orange, Wake, Person, and Granville Counties, as well as plantations in Alabama and Mississippi. This industrious family originally migrated to the Piedmont of North Carolina from neighboring Virginia, beginning in the mid-eighteenth century. He was the fifth child of Rachel and Dudley Bennehan, modest landowners of Irish extraction. Dudley Bennehan died when Richard was only six, and did not leave any of his estate to Richard.

Instead, Richard was apprenticed to a local merchant. In , he moved to Petersburg, Va. Bennehan accepted the offer, and moved to North Carolina in late or early Because of the store’s excellent location and Bennehan’s able management, the business prospered.

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Summary This report outlines recommendations for the clinical use of the three smallpox vaccines stored in the U. Strategic National Stockpile for persons who are exposed to smallpox virus or at high risk for smallpox infection during a postevent vaccination program following an intentional or accidental release of the virus. No absolute contraindications exist for smallpox vaccination in a postevent setting.

However, several relative contraindications exist among persons with certain medical conditions.

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Walk upstairs and be greeted by two master suites that are very spacious one has a gorgeous master bath recently refinished along with a huge walk in closet and the other has a sitting room and a spacious walk in closet. A team of insurance professionals, working together for the benefit of their clients: Stepping inside this stately home will bring you back in time with superb wood craftsmanship two brick fireplaces on main level deep window sills high ceilings a each room is more spacious then the next.

Stepping inside this stately home will bring you back in time with superb wood craftsmanship, two brick fireplaces on main level, deep window sills, high ceilings, a each room is more spacious then the next. Kitchen has been recently upgraded with surrounding custom cabinetry, granite counter tops, light fixtures, and a delightful breakfast area. Information being provided is for consumers’ personal, non-commercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing.

This information is provided for general informational purposes only and should not be relied on in making any home-buying decisions.

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